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RADIO IMMUNOASSAY THYROIDS & AUTOIMMUNE

T3-Uptake

Immunoradiometric assay for quantitative determination of T3 Uptake in human serum or plasma

Summary

  • KFDA Registration No

    17-4557

  • CAT No

    RT10

  • TEST METHOD

    IRMA

  • SAMPLE VOLUME

    20uL

  • INCUBATION TIME

    60'RT

  • STD RANGE

    Qualitative

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Intended Use

Intended Use

Immunoradiometric assay for quantitative determination of T3 Uptake in human serum or plasma

Principle of the Assay

The RIAKEY T3 Uptake RIA tube is based on the determination of unsaturated binding capacity of Thyroxine Binding Globulin (TBG) in human serum or plasma. Reference serum, control samples and patient sera are incubates in anti-T3 monoclonal antibodies coated tubes and 125I-labeled T3 Tracer. The amount of radioactive T3 bound to antibody is indirectly proportional to the amount of unsaturated binding sites on TBG. After a one hour incubation the 125I-T3 are fixed to the test tube wall and the reaction mixture is aspirated or decanted in order to remove free iodine labeled T3 tracer. Radioactivity bound to the reactive sites of the test tube walls is then determines in a gamma counter. The T3 Uptake value of the patient sample’s CPM and the reference samples’ CPM using a predefined percentage T3 Uptake value of the reference serum.

Use Precaution

Be careful when handling all samples, reagents, or devices used in the test as they may be the source of infection.
All reagents, human body samples, etc. are handled at the designated location.

  1. Do not use mixed reagents from different lots.
  2. Do not use reagents beyond the expiration date.
  3. Use distilled water stored in clean container.
  4. Use an individual disposable tip for each sample and reagent, to prevent the possible cross-contamination among the samples.
  5. Store the unused coated tubes at 2~8ºC in the appropriate bags with silica gel and accurately sealed.
  6. If large quantity of assay would be performed at one time, there might be substantial time variation between 60 tubes at one time to minimize time variation. Also, do not exceed 10 minutes for entire pipetting.
  7. Wear disposable globes while handling the kit reagents and wash hands thoroughly afterwards.
  8. Do not pipette by mouth.
  9. Do not smoke, eat or drink in areas where specimens or kit reagents are handle.
  10. Handle samples, reagents and loboratory equipments used for assy with extreme care, as they may potentially contain infectious agents.
  11. When samples or reagents happen to be split, wash carefully with a 3% sodium hypochlorite solution.
  12. Dispose of this cleaning liquid and also such used washing cloth or tissue paper with care, as they may also contain infectious agents.
  13. Avoid microbial contamination when the reagent vial be eventually opend or the contents be handled.
  14. Use only for IN VITRO.
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