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RADIO IMMUNOASSAY THYROIDS & AUTOIMMUNE

Anti-Tg

RIAKEY TG Ab RIA Tube II is an in vitro diagnostic assay for the quantitative determination of Anti-Thyroglobulin (Anti-TG)) autoantibodies in human serum.

Summary

  • KFDA Registration No

    16-969

  • CAT No

    RT12

  • TEST METHOD

    IRMA

  • SAMPLE VOLUME

    20uL

  • INCUBATION TIME

    60'+30'RT

  • STD RANGE

    0 - 2000 IU/ml

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Intended Use

Intended Use

RIAKEY TG Ab RIA Tube II is an in vitro diagnostic assay for the quantitative determination of Anti-Thyroglobulin (Anti-TG)) autoantibodies in human serum

Introduction

Thyroid autoimmunity is more frequently registered in women. Antibody prevalence in women increases with age, rising from approximately 10% at the age of 18-24 up to 30% at the age of 55-65 for Tg Ab and from 15% at the age of 18-24 up to 24% at the age of 55-65 for TMAb.

Principle of the Assay

The present method is based on a competitive radioimmunoassay (RIA). During the incubation, the monoclonal anti-Tg antibody on solid phase competes with the sample/calibrator anti-Tg autoantibodies for the specific sites of the Iodine125 labelled Tg (tracer). After aspiration and washing, the radioactivity in the tubes is measured in a gamma counter. The degree of binding will be inversely proportional to the sample anti-Tg autoantibody concentration. The calibrators of RIAKEY TG Ab RIA Tube are calibrated against the TgAb international reference preparation MRC65/93. 


Use Precaution

Be careful when handling all samples, reagents, or devices used in the test as they may be the source of infection.
All reagents, human body samples, etc. are handled at the designated location.

  1. Do not use mixed reagents from different lots.
  2. Do not use reagents beyond the expiration date.
  3. Use distilled water stored in clean container.
  4. Use an individual disposable tip for each sample and reagent, to prevent the possible cross-contamination among the samples.
  5. Store the unused coated tubes at 2~8ºC in the appropriate bags with silica gel and accurately sealed.
  6. If large quantity of assay would be performed at one time, there might be substantial time variation between 60 tubes at one time to minimize time variation. Also, do not exceed 10 minutes for entire pipetting.
  7. Wear disposable globes while handling the kit reagents and wash hands thoroughly afterwards.
  8. Do not pipette by mouth.
  9. Do not smoke, eat or drink in areas where specimens or kit reagents are handle.
  10. Handle samples, reagents and loboratory equipments used for assy with extreme care, as they may potentially contain infectious agents.
  11. When samples or reagents happen to be split, wash carefully with a 3% sodium hypochlorite solution.
  12. Dispose of this cleaning liquid and also such used washing cloth or tissue paper with care, as they may also contain infectious agents.
  13. Avoid microbial contamination when the reagent vial be eventually opend or the contents be handled.
  14. Use only for IN VITRO.
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